| Item type |
Departmental Bulletin Paper(1) |
| 公開日 |
2024-04-12 |
| タイトル |
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|
タイトル |
Gene expression of SHARP-2 is induced by transforming growth factor beta via multiple pathways in HuH-7 hepatoma cells |
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言語 |
en |
| タイトル |
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|
タイトル |
HuH-7肝癌細胞において、SHARP-2遺伝子の発現は、トランスフォーミング成長因子βにより様々な経路を介して誘導される |
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言語 |
ja |
| 言語 |
|
|
言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
SHARP-2 |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
TGF-β |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gene regulation |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
signaling pathway |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HuH-7 hepatoma cells |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| 著者 |
YAMADA, Kazuya
AOKI, Masato
KOITABASHI, Haruka
MISAKI, Toshinori
SHIOTANI, Kazusa
HONZAWA, Satoru
KOBAYASHI, Miho
KOIDE, Kasumi
WADA, Manami
KOSUDA , Yuka
NISHIZAWA, Anna
TAKAGI, Nanami
MIYASHITA, Mika
ISSHI, Yuka
TSUKADA, Akiko
TAKAGI, Katsuhiro
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Transforming growth factor-β (TGF-β) inhibits cell proliferation and promotes cell differentiation. In human HuH-7 hepatocellular carcinoma cells, TGF-β reduces the expression of the α-fetoprotein (AFP) gene, a marker molecule for hepatocellular carcinoma cells. In this case, the amount of AT motif-binding factor-1 (ATBF1) mRNA, which encodes a transcriptional repressor of the AFP gene, was not changed. This suggests the involvement of factors that are induced by TGF-β and interact with ATBF1. The enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcriptional repressor and an interacting protein of ATBF1. In this study, we examined whether TGF-β regulates the expression of the SHARP-2 gene in HuH-7 cells. TGF-β induced the SHARP-2 mRNA level within 1 hr. Next, to explore the signaling pathway, HuH-7 cells were pretreated with various inhibitors and then treated with TGF-β. Inhibitors of TGF-β type I receptor, phosphoinositide 3-kinase, protein kinase C, Jun N-terminal kinase, mitogen-activated protein kinase, and RNA polymerase II completely or partially diminished the induction of the SHARP-2 mRNA level by TGF-β. Finally, a reporter plasmid containing from -3,700 to +265 region of the SHARP-2 gene were transfected into HuH-7 cells and treated with TGF-β, but no change in promoter activity was observed. Thus, we concluded that TGF-β increased the level of SHARP-2 mRNA via multiple pathways at the transcription level of the SHARP-2 gene and that SHARP-2 is a candidate protein mediating TGF-β-repression of the AFP gene. |
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言語 |
en |
| 書誌情報 |
ja : 松本大学研究紀要
en : The Journal of Matsumoto University
号 22,
p. 97-106,
発行日 2024-03-15
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| 出版者 |
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出版者 |
学校法人松商学園松本大学・松本大学松商短期大学部 |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1348-0618 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11824385 |
matsu_kiyo_22-08.pdf (568 KB)
