{"created":"2024-04-08T03:00:10.886992+00:00","id":2000142,"links":{},"metadata":{"_buckets":{"deposit":"e0478f0a-e5eb-4085-a8c8-a56b1b2d5f33"},"_deposit":{"created_by":18,"id":"2000142","owner":"18","owners":[18],"pid":{"revision_id":0,"type":"depid","value":"2000142"},"status":"published"},"_oai":{"id":"oai:matsumoto-u.repo.nii.ac.jp:02000142","sets":["1:1712131276796"]},"author_link":[],"control_number":"2000142","item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"106","bibliographicPageStart":"97","bibliographic_titles":[{"bibliographic_title":"松本大学研究紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"The Journal of Matsumoto University","bibliographic_titleLang":"en"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transforming growth factor-β (TGF-β) inhibits cell proliferation and promotes cell differentiation. In human HuH-7 hepatocellular carcinoma cells, TGF-β reduces the expression of the α-fetoprotein (AFP) gene, a marker molecule for hepatocellular carcinoma cells. In this case, the amount of AT motif-binding factor-1 (ATBF1) mRNA, which encodes a transcriptional repressor of the AFP gene, was not changed. This suggests the involvement of factors that are induced by TGF-β and interact with ATBF1. The enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcriptional repressor and an interacting protein of ATBF1. In this study, we examined whether TGF-β regulates the expression of the SHARP-2 gene in HuH-7 cells. TGF-β induced the SHARP-2 mRNA level within 1 hr. Next, to explore the signaling pathway, HuH-7 cells were pretreated with various inhibitors and then treated with TGF-β. Inhibitors of TGF-β type I receptor, phosphoinositide 3-kinase, protein kinase C, Jun N-terminal kinase, mitogen-activated protein kinase, and RNA polymerase II completely or partially diminished the induction of the SHARP-2 mRNA level by TGF-β. Finally, a reporter plasmid containing from -3,700 to +265 region of the SHARP-2 gene were transfected into HuH-7 cells and treated with TGF-β, but no change in promoter activity was observed. Thus, we concluded that TGF-β increased the level of SHARP-2 mRNA via multiple pathways at the transcription level of the SHARP-2 gene and that SHARP-2 is a candidate protein mediating TGF-β-repression of the AFP gene.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"学校法人松商学園松本大学・松本大学松商短期大学部"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11824385","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-0618","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMADA, Kazuya"}]},{"creatorNames":[{"creatorName":"AOKI, Masato"}]},{"creatorNames":[{"creatorName":"KOITABASHI, Haruka"}]},{"creatorNames":[{"creatorName":"MISAKI, Toshinori"}]},{"creatorNames":[{"creatorName":"SHIOTANI, Kazusa"}]},{"creatorNames":[{"creatorName":"HONZAWA, Satoru"}]},{"creatorNames":[{"creatorName":"KOBAYASHI, Miho"}]},{"creatorNames":[{"creatorName":"KOIDE, Kasumi"}]},{"creatorNames":[{"creatorName":"WADA, Manami"}]},{"creatorNames":[{"creatorName":"KOSUDA , Yuka"}]},{"creatorNames":[{"creatorName":"NISHIZAWA, Anna"}]},{"creatorNames":[{"creatorName":"TAKAGI, Nanami"}]},{"creatorNames":[{"creatorName":"MIYASHITA, Mika"}]},{"creatorNames":[{"creatorName":"ISSHI, Yuka"}]},{"creatorNames":[{"creatorName":"TSUKADA, Akiko"}]},{"creatorNames":[{"creatorName":"TAKAGI, Katsuhiro"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-04-12"}],"filename":"matsu_kiyo_22-08.pdf","filesize":[{"value":"568 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://matsumoto-u.repo.nii.ac.jp/record/2000142/files/matsu_kiyo_22-08.pdf"},"version_id":"1f5fde35-8c53-473f-a28b-e34aac0c3cb0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SHARP-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TGF-β","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene regulation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"signaling pathway","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HuH-7 hepatoma cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gene expression of SHARP-2 is induced by transforming growth factor beta via multiple pathways in HuH-7 hepatoma cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene expression of SHARP-2 is induced by transforming growth factor beta via multiple pathways in HuH-7 hepatoma cells","subitem_title_language":"en"},{"subitem_title":"HuH-7肝癌細胞において、SHARP-2遺伝子の発現は、トランスフォーミング成長因子βにより様々な経路を介して誘導される","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"18","path":["1712131276796"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-12"},"publish_date":"2024-04-12","publish_status":"0","recid":"2000142","relation_version_is_last":true,"title":["Gene expression of SHARP-2 is induced by transforming growth factor beta via multiple pathways in HuH-7 hepatoma cells"],"weko_creator_id":"18","weko_shared_id":-1},"updated":"2024-04-12T07:57:55.600257+00:00"}