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SHARP-2相互作用タンパク質の同定と特性
https://matsumoto-u.repo.nii.ac.jp/records/1369
https://matsumoto-u.repo.nii.ac.jp/records/13692e89ce13-68d2-499f-9824-79891ec6d196
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
matsu_kiyo_20-09.pdf (431.0 kB)
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| Item type | Departmental Bulletin Paper(1) | |||||||||||||||||||||||||||||||||||
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| 公開日 | 2022-05-14 | |||||||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||||||
| タイトル | SHARP-2相互作用タンパク質の同定と特性 | |||||||||||||||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||||||||||||||||||||
| 著者 |
山田, 一哉
× 山田, 一哉
× 三島, 歩実
× 花岡, 由紀奈
× 三崎, 紀展
× 上條, 茜
× 坂本, 那津子
× 茅野, 友加里
× 堀内, 涼香
× 近藤, 晴菜
× 長井, 朱里
× 丸山, ゆきの
× 山根, 優奈
× 田野口, 愛華
× 塚田, 晃子
× 髙木, 勝広
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| 抄録 | ||||||||||||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||||||||||
| 内容記述 | The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcriptional repressor. Many transcription factors interact with other proteins to form complex transcriptional regulation networks. To identify a SHARP-2-interacting protein(s), we screened a rat liver cDNA library using a yeast two-hybrid system. Approximately 3.5×107 independent clones were screened and 29 positive clones were obtained. Of these, multiple clones contained AT motif-binding factor-1 (ATBF1) and Sex-determining region Y-box 6 (Sox6) which both were transcription factors. We then focused on and analyzed physical protein-protein interaction of SHARP-2 with ATBF1 and Sox6. First, minimal interaction domain of ATBF1 and Sox6 with the SHARP-2 was determined. Amino acid residues between 3,271 and 3,379 of the ATBF1 and those between 201 and 362 of Sox6 were required for an interaction with SHARP-2, respectively. We then examined which domain of SHARP-2 is required for interactions with ATBF1 and Sox6. Amino acid residues between 301 and 355 of the SHARP-2 interacted with the ATBF1 and those between 384 and 397 of the SHARP-2 interacted with the Sox6. Thus, we concluded that both the ATBF1 and Sox6 were identified as the SHARP-2-interacting protein and that the SHARP-2 interacted with them using different domains. | |||||||||||||||||||||||||||||||||||
| 書誌情報 |
松本大学研究紀要 en : The Journal of Matsumoto University 巻 20, p. 105-115, 発行日 2022-03-10 |
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| 出版者 | ||||||||||||||||||||||||||||||||||||
| 出版者 | 学校法人松商学園松本大学 | |||||||||||||||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||||||
| 収録物識別子 | 1348-0618 | |||||||||||||||||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||||||||||
| 収録物識別子 | AA11824385 | |||||||||||||||||||||||||||||||||||
| フォーマット | ||||||||||||||||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||
