{"created":"2023-06-20T15:14:31.714164+00:00","id":1369,"links":{},"metadata":{"_buckets":{"deposit":"2393599f-50c0-4527-a0dc-7c9e80f176cf"},"_deposit":{"created_by":7,"id":"1369","owners":[7],"pid":{"revision_id":0,"type":"depid","value":"1369"},"status":"published"},"_oai":{"id":"oai:matsumoto-u.repo.nii.ac.jp:00001369","sets":["1:138"]},"author_link":["1022","1010","1012","1013","1019","1015","1021","1018","1014","1023","1024","1016","1011","1020","1017"],"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"115","bibliographicPageStart":"105","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"松本大学研究紀要"},{"bibliographic_title":"The Journal of Matsumoto University","bibliographic_titleLang":"en"}]}]},"item_2_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcriptional repressor. Many transcription factors interact with other proteins to form complex transcriptional regulation networks. To identify a SHARP-2-interacting protein(s), we screened a rat liver cDNA library using a yeast two-hybrid system. Approximately 3.5×107  independent clones were screened and 29 positive clones were obtained. Of these, multiple clones contained AT motif-binding factor-1 (ATBF1) and Sex-determining region Y-box 6 (Sox6) which both were transcription factors. We then focused on and analyzed physical protein-protein interaction of SHARP-2 with ATBF1 and Sox6. First, minimal interaction domain of ATBF1 and Sox6 with the SHARP-2 was determined. Amino acid residues between 3,271 and 3,379 of the ATBF1 and those between 201 and 362 of Sox6 were required for an interaction with SHARP-2, respectively. We then examined which domain of SHARP-2 is required for interactions with ATBF1 and Sox6. Amino acid residues between 301 and 355 of the SHARP-2 interacted with the ATBF1 and those between 384 and 397 of the SHARP-2 interacted with the Sox6. Thus, we concluded that both the ATBF1 and Sox6 were identified as the SHARP-2-interacting protein and that the SHARP-2 interacted with them using different domains.","subitem_description_type":"Abstract"}]},"item_2_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"学校法人松商学園松本大学"}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11824385","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-0618","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山田, 一哉"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三島, 歩実"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"花岡, 由紀奈"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三崎, 紀展"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上條, 茜"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坂本, 那津子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"茅野, 友加里"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"堀内, 涼香"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"近藤, 晴菜"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"長井, 朱里"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"丸山, ゆきの"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山根, 優奈"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田野口, 愛華"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"塚田, 晃子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"髙木, 勝広"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-14"}],"displaytype":"detail","filename":"matsu_kiyo_20-09.pdf","filesize":[{"value":"431.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"matsu_kiyo_20-09.pdf","url":"https://matsumoto-u.repo.nii.ac.jp/record/1369/files/matsu_kiyo_20-09.pdf"},"version_id":"84d1a632-a6d9-441c-bba8-4eff329aac08"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"SHARP-2相互作用タンパク質の同定と特性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"SHARP-2相互作用タンパク質の同定と特性"}]},"item_type_id":"2","owner":"7","path":["138"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-14"},"publish_date":"2022-05-14","publish_status":"0","recid":"1369","relation_version_is_last":true,"title":["SHARP-2相互作用タンパク質の同定と特性"],"weko_creator_id":"7","weko_shared_id":7},"updated":"2023-06-20T15:23:11.197604+00:00"}