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SHARP-2相互作用タンパク質の同定と特性
https://matsumoto-u.repo.nii.ac.jp/records/1369
https://matsumoto-u.repo.nii.ac.jp/records/13692e89ce13-68d2-499f-9824-79891ec6d196
名前 / ファイル | ライセンス | アクション |
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matsu_kiyo_20-09.pdf (431.0 kB)
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Item type | Departmental Bulletin Paper(1) | |||||
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公開日 | 2022-05-14 | |||||
タイトル | ||||||
タイトル | SHARP-2相互作用タンパク質の同定と特性 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
山田, 一哉
× 山田, 一哉× 三島, 歩実× 花岡, 由紀奈× 三崎, 紀展× 上條, 茜× 坂本, 那津子× 茅野, 友加里× 堀内, 涼香× 近藤, 晴菜× 長井, 朱里× 丸山, ゆきの× 山根, 優奈× 田野口, 愛華× 塚田, 晃子× 髙木, 勝広 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcriptional repressor. Many transcription factors interact with other proteins to form complex transcriptional regulation networks. To identify a SHARP-2-interacting protein(s), we screened a rat liver cDNA library using a yeast two-hybrid system. Approximately 3.5×107 independent clones were screened and 29 positive clones were obtained. Of these, multiple clones contained AT motif-binding factor-1 (ATBF1) and Sex-determining region Y-box 6 (Sox6) which both were transcription factors. We then focused on and analyzed physical protein-protein interaction of SHARP-2 with ATBF1 and Sox6. First, minimal interaction domain of ATBF1 and Sox6 with the SHARP-2 was determined. Amino acid residues between 3,271 and 3,379 of the ATBF1 and those between 201 and 362 of Sox6 were required for an interaction with SHARP-2, respectively. We then examined which domain of SHARP-2 is required for interactions with ATBF1 and Sox6. Amino acid residues between 301 and 355 of the SHARP-2 interacted with the ATBF1 and those between 384 and 397 of the SHARP-2 interacted with the Sox6. Thus, we concluded that both the ATBF1 and Sox6 were identified as the SHARP-2-interacting protein and that the SHARP-2 interacted with them using different domains. | |||||
書誌情報 |
松本大学研究紀要 en : The Journal of Matsumoto University 巻 20, p. 105-115, 発行日 2022-03-10 |
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出版者 | ||||||
出版者 | 学校法人松商学園松本大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1348-0618 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11824385 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |